RNA-Seq as an Effective Tool for Modern Transcriptomics, A Review-based Study

Transcriptome analysis is a useful method for identification and understanding genes. Finding genes that are differentially expressed between conditions crucial aspect of transcriptomics. The discovery RNA seq has been revolutionized next-generation sequencing technology. fact does not requires gene probes provides precise measure expression over much wider range proved its credibility other common techniques. profile transcriptome data stored in database could be accessed freely. During short read mapping to the reference (the set all known transcript sequences species) or genome database, variety search tools alignment methods become visible. There applications help align reads generated by fragment sequencing. study aided quantifying transcriptome. gives information regarding alternative splicing isoforms, addition differential expression.